Sofosbuvir

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

HPLC Method for Sofosbuvir on Primesep B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sofosbuvir

Sofosbuvir is an oral antiviral medication used to treat hepatitis C virus (HCV) infection. It is a nucleotide analog inhibitor that targets the HCV NS5B RNA polymerase, preventing viral replication. Often combined with other antiviral drugs, sofosbuvir has revolutionized HCV treatment by offering a shorter duration of therapy, higher cure rates, and minimal resistance, with fewer side effects compared to older treatments.

Sofosbuvir can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 260 nm.

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.