HPLC Method for Sofosbuvir on Primesep B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sofosbuvir
Sofosbuvir is an oral antiviral medication used to treat hepatitis C virus (HCV) infection. It is a nucleotide analog inhibitor that targets the HCV NS5B RNA polymerase, preventing viral replication. Often combined with other antiviral drugs, sofosbuvir has revolutionized HCV treatment by offering a shorter duration of therapy, higher cure rates, and minimal resistance, with fewer side effects compared to older treatments.
Sofosbuvir can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 260 nm.
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm |
| LOD | 70.3 ppb |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | Nucleotide Analog Prodrug |
| Analyzing Compounds | Sofosbuvir |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
