Pyrantel structural formula

UV absorption spectrum of Pyrantel

CAS Number15686-83-6
Molecular FormulaC11H14N2S
Molecular Weight206.310
  • Pyrantel
  • 1-Methyl-2-[(E)-2-(thiophen-2-yl)ethenyl]-1,4,5,6-tetrahydropyrimidine
  • Pyrimidine, 1,4,5,6-tetrahydro-1-methyl-2-[(E)-2-(2-thienyl)ethenyl]-
  • 15686-83-6
  • EINECS 239-774-1
  • Pyrequan
  • Strongid
  • E-1,4,5,6-Tetrahydro-1-methyl-2-(2-(2-thienyl)vinyl)pyrimidine
  • Pirantel
  • Pirantele
  • Pyrantelum
  • UNII-4QIH0N49E7


HPLC method for Separation Pamoic Acid, Ivermectin, Pyrantel on Primesep 100 Column

February 2, 2024

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pamoic acid, Ivermectin B1a, Pyrantel on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC method for Separation Pamoic Acid, Ivermectin,Pyrantel on Primesep 100 ColumnJ
HPLC Method for Analysis of Pamoic acid, Ivermectin B1a, Pyrantel on Primesep 100 Column by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Pamoic acid, Ivermectin B1a, Pyrantel

Pamoic Acid:

  • Pamoic acid is a compound used in pharmaceuticals, particularly as a salt form.
  • Its salt, such as pamoate salts, is often used to improve the solubility and stability of certain drugs.
  • Pamoic acid is commonly used in the formulation of drugs to enhance their pharmacokinetic properties.


  • Ivermectin is an antiparasitic medication that belongs to the class of drugs called avermectins.
  • It is widely used to treat parasitic infections, including certain types of roundworm infections and scabies.
  • Ivermectin works by interfering with the nervous system of parasites, leading to their paralysis and eventual death.


  • Pyrantel is an anthelmintic or deworming medication.
  • It is commonly used to treat infections caused by roundworms and hookworms.
  • Pyrantel works by causing neuromuscular blockade in the parasites, leading to their expulsion from the digestive tract.

Pamoic Acid, Ivermectin, Pyrantel be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 245 and 315 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 60%
BufferH2SO4 -0.2%
Flow Rate1.0 ml/min
DetectionUV 245, 315 nm
SamplesIvermectin – 0.25 mg/ml
Pyrantel Pamoate – 0.25 mg/ml
Injection volume2 µl
LOD*1.  2.6 ppb (245 nm)
2. 18 ppb (245 nm)
3. 29 ppb (315 nm)
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Analyzing CompoundsPamoic acid, Ivermectin B1a, Pyrantel

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Ivermectin B1a
Pamoic acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.

Separation of Pyrantel on Newcrom R1 HPLC column

February 16, 2018
Separation of Pyrantel on Newcrom C18 HPLC column

Pyrantel can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: or by phone: 847-229-2629.