HPLC method for Separation Pamoic Acid, Ivermectin, Pyrantel on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pamoic acid, Ivermectin B1a, Pyrantel on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC method for Separation Pamoic Acid, Ivermectin,Pyrantel on Primesep 100 ColumnJ
HPLC Method for Analysis of Pamoic acid, Ivermectin B1a, Pyrantel on Primesep 100 Column by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Pamoic acid, Ivermectin B1a, Pyrantel

Pamoic Acid:

  • Pamoic acid is a compound used in pharmaceuticals, particularly as a salt form.
  • Its salt, such as pamoate salts, is often used to improve the solubility and stability of certain drugs.
  • Pamoic acid is commonly used in the formulation of drugs to enhance their pharmacokinetic properties.


  • Ivermectin is an antiparasitic medication that belongs to the class of drugs called avermectins.
  • It is widely used to treat parasitic infections, including certain types of roundworm infections and scabies.
  • Ivermectin works by interfering with the nervous system of parasites, leading to their paralysis and eventual death.


  • Pyrantel is an anthelmintic or deworming medication.
  • It is commonly used to treat infections caused by roundworms and hookworms.
  • Pyrantel works by causing neuromuscular blockade in the parasites, leading to their expulsion from the digestive tract.

Pamoic Acid, Ivermectin, Pyrantel be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 245 and 315 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 60%
BufferH2SO4 -0.2%
Flow Rate1.0 ml/min
DetectionUV 245, 315 nm
SamplesIvermectin – 0.25 mg/ml
Pyrantel Pamoate – 0.25 mg/ml
Injection volume2 µl
LOD*1.  2.6 ppb (245 nm)
2. 18 ppb (245 nm)
3. 29 ppb (315 nm)
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Analyzing CompoundsPamoic acid, Ivermectin B1a, Pyrantel

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Ivermectin B1a
Pamoic acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.