| CAS Number | 328-42-7 |
|---|---|
| Molecular Formula | C4H4O5 |
| Molecular Weight | 132.07 |
| InChI Key | KHPXUQMNIQBQEV-UHFFFAOYSA-N |
| LogP | -0.6 |
| Synonyms |
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Applications:
HPLC Method for the determination of Oxaloacetic and Oxalic acids on Newcrom BH
November 5, 2021
HPLC Method for Oxalic Acid, Oxaloacetic acid (oxalacetic acid, OAA) on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Oxalic Acid, Oxaloacetic acid (oxalacetic acid, OAA).
Oxaloacetic acid, when in the form of its conjugate base oxaloacetate, is a metabolic intermediate in many biological processes, such as the urea and citric acid cycles, among others. Oxalic acid (also known as oxalate) is a naturally occurring compound that is found in many leafy greens, fruits, and nuts. It can also be used for whitening teeth and removing rust.
Multi-charged molecules, such as oxaloacetate and oxalate, generally have a tendency to exhibit poor peak shape on reverse-phase HPLC columns, where they show significant tailing. This compound can be retained with great peak shape on a Newcrom BH mixed-mode column.Oxaloacetic acid and oxalic acid can be measured at low UV. Using a Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer, oxaloacetic acid and oxalic acid can be retained, separated, and UV detected at 200 nm with precise resolution.
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Acids |
| Analyzing Compounds | Oxalic Acid, Oxaloacetic acid (oxalacetic acid, OAA) |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Oxaloacetic acid (oxalacetic acid, OAA)
