Oxaloacetic acid, when in the form of its conjugate base oxaloacetate, is a metabolic intermediate in many biological processes, such as the urea and citric acid cycles, among others. Oxalic acid (also known as oxalate) is a naturally occurring compound that is found in many leafy greens, fruits, and nuts. It can also be used for whitening teeth and removing rust.
Multi-charged molecules, such as oxaloacetate and oxalate, generally have a tendency to exhibit poor peak shape on reverse-phase HPLC columns, where they show significant tailing. This compound can be retained with great peak shape on a Newcrom BH mixed-mode column.Oxaloacetic acid and oxalic acid can be measured at low UV. Using a Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer, oxaloacetic acid and oxalic acid can be retained, separated, and UV detected at 200 nm with precise resolution.