| CAS Number | 12650-69-0 |
|---|---|
| Molecular Formula | C26H44O9 |
| Molecular Weight | 500.629 |
| InChI Key | MINDHVHHQZYEEK-HBBNESRFSA-N |
| LogP | 2.59 |
| Synonyms |
|
Applications:
LC-MS Method for Analysis of Mupirocin on Newcrom BH Column
September 14, 2023
HPLC Method for Analysis of Mupirocin on Newcrom BH Column by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Mupirocin is an antibiotic ointment commonly used for the treatment of bacterial skin infections.
Mechanism of Action: Mupirocin works by inhibiting the bacterial enzyme isoleucyl-tRNA synthetase. This disruption prevents the bacteria from making necessary proteins, leading to its death.
Usage:
Topical Mupirocin: Commonly prescribed for skin infections caused by Staphylococcus and Streptococcus bacteria. It is effective against both methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA).
It is also used to eliminate nasal colonization of MRSA in some healthcare settings.
Formulation: Mupirocin is typically available as a topical ointment or cream. There is also a nasal formulation for specific uses.
Mupirocin can be retained, separated, and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and Ammonium Formate. This analytical method can be detected with high resolution and peak symmetry with many evaporative detection methods, including Evaporative Light Scattering Detection (ELSD), Charged Aerosol Detector (CAD), and Electrospray Ionization (ESI) for Mass Spectrometry (MS).
High Performance Liquid Chromatography (HPLC) Method for Analyses of Michler’s ketone
Condition
| Column | Newcrom BH, 3.2 x 100 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeCN – 20-70%, 10 min |
| Buffer | Formic acid – 0.2% |
| Flow Rate | 0.5 ml/min |
| Peak Retention Time | 7.58 min |
| Detection | (-) ESI , SIM 499 – |
| Spray Voltage: | 1.5 kV |
| Nebulizing gas: | 1.5 L/min |
| Drying gas: | 15 L/min |
| DL temp: | 250 ˚C |
| Heat Block: | 400 ˚C |
Description
| Class of Compounds | Drug, antibiotic |
| Analyzing Compounds | Mupirocin |
Application Column
Newcrom BH
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
HPLC Method for Analysis of Mupirocin in Pharmaceutical Dosage Form on Newcrom BH Column
September 14, 2023
HPLC Method for Analysis of Mupirocin in Pharmaceutical Dosage Form on Newcrom BH Column by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Mupirocin is an antibiotic ointment commonly used for the treatment of bacterial skin infections. Here are some key points about mupirocin:
Mechanism of Action: Mupirocin works by inhibiting the bacterial enzyme isoleucyl-tRNA synthetase. This disruption prevents the bacteria from making necessary proteins, leading to its death.
Usage:
Topical Mupirocin: Commonly prescribed for skin infections caused by Staphylococcus and Streptococcus bacteria. It is effective against both methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA).
It is also used to eliminate nasal colonization of MRSA in some healthcare settings.
Formulation: Mupirocin is typically available as a topical ointment or cream. There is also a nasal formulation for specific uses.
Mupirocin can be retained, separated, and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and Ammonium Formate. This analytical method can be detected with high resolution and peak symmetry with many evaporative detection methods, including Evaporative Light Scattering Detection (ELSD), Charged Aerosol Detector (CAD), and Electrospray Ionization (ESI) for Mass Spectrometry (MS).
High Performance Liquid Chromatography (HPLC) Method for Analyses of Michler’s ketone
Condition
| Column | Newcrom BH, 3.2 x 100 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeCN/H2O – 20-50%, 10 min |
| Buffer | Ammonium formate pH 3.0 –20 mM |
| Flow Rate | 0.5 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
Description
| Class of Compounds | Drug, antibiotic |
| Analyzing Compounds | Mupirocin |
Application Column
Newcrom BH
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Separation of Mupirocin on Newcrom R1 HPLC column
February 16, 2018
Mupirocin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options