HPLC Method for Analysis of Mupirocin on Newcrom BH Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Mupirocin.
Mupirocin is an antibiotic ointment with the chemical formula C26H44O9. Commonly prescribed for skin infections caused by Staphylococcus and Streptococcus bacteria. It is effective against both methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA). It is also used to eliminate nasal colonization of MRSA in some healthcare settings. Mupirocin works by inhibiting the bacterial enzyme isoleucyl-tRNA synthetase. This disruption prevents the bacteria from making necessary proteins, leading to its death.
Mupirocin can be retained, separated, and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and Ammonium Formate. This analytical method can be detected with high resolution and peak symmetry with many evaporative detection methods, including Evaporative Light Scattering Detection (ELSD), Charged Aerosol Detector (CAD), and Electrospray Ionization (ESI) for Mass Spectrometry (MS).
Condition
| Column | Newcrom BH, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 20-70%, 10 min |
| Buffer | Formic acid – 0.2% |
| Flow Rate | 0.5 ml/min |
| Peak Retention Time | 7.58 min |
| Detection | (-) ESI , SIM 499 – |
| Spray Voltage: | 1.5 kV |
| Nebulizing gas: | 1.5 L/min |
| Drying gas: | 15 L/min |
| DL temp: | 250 ˚C |
| Heat Block: | 400 ˚C |
Description
| Class of Compounds | Drug, antibiotic |
| Analyzing Compounds | Mupirocin |
Application Column
Newcrom BH
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
