LC-MS Method for Analysis of Mupirocin on Newcrom BH Column

HPLC Method for Analysis of Mupirocin on Newcrom BH Column by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC - MS Method for Analysis of Mupirocin on Newcrom BH Column by SIELC Technologies

Mupirocin is an antibiotic ointment commonly used for the treatment of bacterial skin infections.

Mechanism of Action: Mupirocin works by inhibiting the bacterial enzyme isoleucyl-tRNA synthetase. This disruption prevents the bacteria from making necessary proteins, leading to its death.


Topical Mupirocin: Commonly prescribed for skin infections caused by Staphylococcus and Streptococcus bacteria. It is effective against both methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA).
It is also used to eliminate nasal colonization of MRSA in some healthcare settings.
Formulation: Mupirocin is typically available as a topical ointment or cream. There is also a nasal formulation for specific uses.

Mupirocin can be retained, separated, and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and Ammonium Formate. This analytical method can be detected with high resolution and peak symmetry with many evaporative detection methods, including Evaporative Light Scattering Detection (ELSD), Charged Aerosol Detector (CAD), and Electrospray Ionization (ESI) for Mass Spectrometry (MS).

High Performance Liquid Chromatography (HPLC) Method for Analyses of Michler’s ketone


ColumnNewcrom BH, 3.2 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 20-70%, 10 min
Buffer Formic acid – 0.2%
Flow Rate0.5 ml/min
Peak Retention Time7.58 min
Detection(-) ESI , SIM 499 –
Spray Voltage: 1.5 kV
Nebulizing gas: 1.5 L/min
Drying gas: 15 L/min
 DL temp: 250 ˚C
Heat Block: 400 ˚C


Class of CompoundsDrug, antibiotic
Analyzing CompoundsMupirocin

Application Column

Newcrom BH

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.