| CAS Number | 90-94-8 |
|---|---|
| Molecular Formula | C17H20N2O |
| Molecular Weight | 268.360 |
| InChI Key | VVBLNCFGVYUYGU-UHFFFAOYSA-N |
| LogP | 3.87 |
| Synonyms |
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Applications:
HPLC-MS Method for Analysis of Michler’s ketone on Primesep 200 Column
August 18, 2023
High Performance Liquid Chromatography (HPLC) Method for Analysis of Michler’s ketone on Primesep 200 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
Michler’s ketone (4,4′-bis(dimethylamino)benzophenone) is a chemical compound used primarily in the synthesis of dyes and pigments. It has the chemical formula C17H17N3O2. The compound is named after the German chemist Viktor Michler, who first prepared it.
Michler’s ketone is synthesized by condensing two equivalents of dimethylaniline with one equivalent of phosgene. The reaction involves the formation of a carbonyl group (C=O) that links two dimethylaniline moieties. The resulting structure features two aromatic rings (benzene rings) connected by a carbonyl group, with dimethylamino groups attached to each aromatic ring.
Michler’s ketone is a valuable intermediate in the dye industry, where it is used to prepare various triarylmethane dyes, including Malachite Green, Methyl Violet, and Crystal Violet. The strong electron-donating properties of the dimethylamino groups make it a useful compound for synthesizing dyes with vibrant and stable colors.
Michler’s ketone can be retained, and analyzed on a Primesep 200 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a formic acid as a buffer. This analysis method can be detected in the UV regime at 371 nm.
High Performance Liquid Chromatography (HPLC) Method for Analyses of Michler’s ketone
Condition
| Column | Primesep 200, 2.1 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 0.2 ml/min |
| Detection | UV 371 nm, LCMS SIM 269+ |
| Peak Retention Time | 7.17 min |
Description
| Class of Compounds | Diaryl ketones |
| Analyzing Compounds | Michler’s ketone |
Application Column
Primesep 200
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
HPLC Method for Analysis of Michler’s ketone on Primesep 100 Column
August 18, 2023
High Performance Liquid Chromatography (HPLC) Method for Analysis of Michler’s ketone on Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
Michler’s ketone (4,4′-bis(dimethylamino)benzophenone) is a chemical compound used primarily in the synthesis of dyes and pigments. It has the chemical formula C17H17N3O2. The compound is named after the German chemist Viktor Michler, who first prepared it.
Michler’s ketone is synthesized by condensing two equivalents of dimethylaniline with one equivalent of phosgene. The reaction involves the formation of a carbonyl group (C=O) that links two dimethylaniline moieties. The resulting structure features two aromatic rings (benzene rings) connected by a carbonyl group, with dimethylamino groups attached to each aromatic ring.
Michler’s ketone is a valuable intermediate in the dye industry, where it is used to prepare various triarylmethane dyes, including Malachite Green, Methyl Violet, and Crystal Violet. The strong electron-donating properties of the dimethylamino groups make it a useful compound for synthesizing dyes with vibrant and stable colors.
Michler’s ketone can be retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV-Vis regime at 540, 590, and 200 nm.
High Performance Liquid Chromatography (HPLC) Method for Analyses of Pararosaniline, Crystal Violet and Crystal Violet Lactone
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 371 nm |
| Peak Retention Time | 8.37 min |
Description
| Class of Compounds | Diaryl ketones |
| Analyzing Compounds | Michler’s ketone |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
HPLC Method for Separation of Michler’s ketone and Crystal Violet on Primesep 100 Column
August 18, 2023
High Performance Liquid Chromatography (HPLC) Method for Analysis of Michler’s ketone, Crystal Violet on Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
Michler’s ketone (4,4′-bis(dimethylamino)benzophenone) is a chemical compound used primarily in the synthesis of dyes and pigments. It has the chemical formula C17H17N3O2. The compound is named after the German chemist Viktor Michler, who first prepared it.
Michler’s ketone is synthesized by condensing two equivalents of dimethylaniline with one equivalent of phosgene. The reaction involves the formation of a carbonyl group (C=O) that links two dimethylaniline moieties. The resulting structure features two aromatic rings (benzene rings) connected by a carbonyl group, with dimethylamino groups attached to each aromatic ring.
Michler’s ketone is a valuable intermediate in the dye industry, where it is used to prepare various triarylmethane dyes, including Malachite Green, Methyl Violet, and Crystal Violet. The strong electron-donating properties of the dimethylamino groups make it a useful compound for synthesizing dyes with vibrant and stable colors.
Michler’s ketone and Crystal Violet can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV-Vis regime at 540, 590, and 200 nm.
High Performance Liquid Chromatography (HPLC) Method for Analyses of Michler’s ketone, Crystal Violet
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 80/20% |
| Buffer | Gr H3PO4 – 0.2-0.4%, 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV 371, Vis 590 nm |
| Peak Retention Time | 3.71, 8.57 min |
Description
| Class of Compounds | Dyes |
| Analyzing Compounds | Michler’s ketone, Crystal Violet |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Michler’s ketone
Separation of Michler’s ketone on Newcrom R1 HPLC column
February 16, 2018
Michler’s ketone can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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