| CAS Number | 83-43-2 |
|---|---|
| Molecular Formula | C22H30O5 |
| Molecular Weight | 374.477 |
| InChI Key | VHRSUDSXCMQTMA-PJHHCJLFSA-N |
| LogP | 1.86 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Methylprednisolone on Primesep B Column
January 29, 2024
HPLC Method for Analysis of Methylprednisolone on Primesep B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Methylprednisolone
Methylprednisolone is a synthetic corticosteroid with anti-inflammatory and immunosuppressive properties. It is a derivative of prednisolone and, like prednisone, belongs to the class of glucocorticoids. Methylprednisolone is used for various medical conditions to reduce inflammation and modify the immune response.
Methylprednisolone can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV 245 nm
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 35% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 245 nm |
| Class of Compounds | Corticosteroids |
| Analyzing Compounds | Methylprednisolone |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
HPLC Method for Separating Corticosteroids such as Prednisone, Prednisolone and Methylprednisolone on Primesep B Column
January 29, 2024
HPLC Method for Prednisone, Prednisolone, Methylprednisolone on Primesep B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Prednisone, Prednisolone, Methylprednisolone
All three compounds have potent anti-inflammatory effects, making them valuable in treating conditions where inflammation plays a role.
Prednisone:
- Mechanism of Action: Prednisone is a synthetic corticosteroid that mimics the action of cortisol, a natural steroid produced by the adrenal glands. It primarily exerts its effects by binding to glucocorticoid receptors and regulating gene expression.
- Medical Uses: It is commonly used to treat inflammatory conditions, autoimmune disorders, allergic reactions, and certain cancers.
Prednisolone:
- Derived Form: Prednisolone is the active form of prednisone. When prednisone is ingested, the liver converts it into prednisolone.
- Mechanism of Action: Similar to prednisone, it has anti-inflammatory and immunosuppressive properties.
Methylprednisolone:
- Potency: Methylprednisolone is considered to be more potent than prednisone.
- Medical Uses: It is used in various conditions, including severe allergic reactions, inflammatory conditions, and as a part of immunosuppressive regimens.
Corticosteroids can be retained, separated, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV at 245 nm
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 35/35% |
| Buffer | H3PO4 -0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 245 nm |
| Class of Compounds | Corticosteroids |
| Analyzing Compounds | Prednisone, Prednisolone, Methylprednisolone |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Prednisolone
Prednisone
Separation of Methylprednisolone on Newcrom R1 HPLC column
February 19, 2018
Methylprednisolone can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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