HPLC Method for Analysis of  Methylprednisolone on Primesep B Column

HPLC Method for Analysis of Methylprednisolone on Primesep B by SIELC Technologies

HPLC Method for Analysis of Methylprednisolone on Primesep B Column by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Methylprednisolone

Methylprednisolone is a synthetic corticosteroid with anti-inflammatory and immunosuppressive properties. It is a derivative of prednisolone and, like prednisone, belongs to the class of glucocorticoids. Methylprednisolone is used for various medical conditions to reduce inflammation and modify the immune response.

 Methylprednisolone can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV 245 nm

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 35%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 245 nm

Class of Compounds
Analyzing CompoundsMethylprednisolone

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.