HPLC Method for Analysis of  Methylprednisolone on Primesep B Column

HPLC Method for Analysis of Methylprednisolone on Primesep B by SIELC Technologies

HPLC Method for Analysis of Methylprednisolone on Primesep B Column by SIELC Technologies

Methylprednisolone is a more potent, synthetic, corticosteroid than prednisone with the chemical formula C22H30O5. It is used in various conditions, including severe allergic reactions, inflammatory conditions, and as a part of immunosuppressive regimens. It is a derivative of prednisolone and, like prednisone, belongs to the class of glucocorticoids. You can find detailed UV spectra of Methylprednisolone and information about its various lambda maxima by visiting the following link.

 Methylprednisolone can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV 245 nm

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 35%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 245 nm

Class of Compounds
Corticosteroids
Analyzing CompoundsMethylprednisolone

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Methylprednisolone
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.