HPLC Method for Separation of Histidine, Histidine Methyl Ester and Histidine lauryl Ester on BIST B+ Column

HPLC Method for Separation of Histidine, Histidine Methyl Ester, and Histidine Lauryl Ester on BIST B+ by SIELC Technologies

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Separation of Histidine, Histidine Methyl Ester and Histidine lauryl Ester on BIST B+ Column by SIELC Technologies.
HPLC Method for Separation of of  Histidine, Histidine Methyl Ester and Histidine lauryl Ester on BIST B+ Column by SIELC Technologies

Histidine is a naturally occurring essential amino acid that the body uses to repair damaged tissue and generate new blood cells. Histidine Methyl Ester is a histidine decarboxylase inhibitor and can be used to synthesize other biological compounds. Histidine Lauryl Ester is another derivative of Histidine. Using SIELC’s newly introduced BIST™ method, Histidine and its derivatives can be retained and separated on a positively charged, anion-exchange BIST™ B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively charged dipeptide to the positively charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Histidine and its derivatives can be UV detected at 220 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Histidine, Histidine Methyl Ester and Histidine lauryl Ester

Condition

ColumnBIST B+, 3.2 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 80 – 50%, 10 min
BufferH2SO4 – 0.2%
Flow Rate0.5 ml/min
DetectionUV 220 nm
Peak Retention Time2.2 min, 7.5 min, 8.9 min

Description

Class of CompoundsAmino acid
Analyzing CompoundsHistidine methyl ester, Histidine

Application Column

BIST B+

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Histidine
Histidine methyl ester
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.