HPLC Separation of Benzoic and 2-naphtalenesulfonic Acids

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Newcrom BH column is used for HPLC separation of weak and strong organic acids using a mixed-mode method.  Benzoic and napthalenesulfonic acids are retained based on polar interaction mode and anion-exchange mode.  The isocratic separation is achieved very quickly on a short 3.2 x 50 mm column in under 3 minutes.  The mobile phase is acetonitrile (ACN) and water with sulfuric acid buffer.  UV detection at 275nm.



Column Newcrom BH, 3.2×50 mm, 3 µm, 100A
Mobile Phase MeCN/H2O – 80/20%
Buffer H2SO4 – 0.2%
Flow Rate 0.5 ml/min
Detection UV 275nm


Class of Compounds Acid
Analyzing Compounds Benzoic Acid,  2-naphtalenesulfonic Acid

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.