HPLC Separation of Benzoic and 2-naphtalenesulfonic Acids

HPLC.cloud View on hplc.cloud Newcrom BH column is used for HPLC separation of weak and strong organic acids using a mixed-mode method.  Benzoic and napthalenesulfonic acids are retained based on polar interaction mode and anion-exchange mode.  The isocratic separation is achieved very quickly on a short 3.2 x 50 mm column in under 3 minutes.  The mobile phase is acetonitrile (ACN) and water with sulfuric acid buffer.  UV detection at 275nm.  

Condition

Column Newcrom BH, 3.2x50 mm, 3 µm, 100A
Mobile Phase MeCN/H2O - 80/20%
Buffer H2SO4 - 0.2%
Flow Rate 0.5 ml/min
Detection UV 275nm

Description

Class of Compounds Acid
Analyzing Compounds Benzoic Acid,  2-naphtalenesulfonic Acid

Application Analytes:
HPLC Separation of Benzoic and 2-naphtalenesulfonic Acids