HPLC Separation of Benzoic and 2-naphtalenesulfonic Acids

HPLC Method for Benzoic Acid, 2-Naphthalenesulfonic Acid on Newcrom BH by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzoic Acid, 2-Naphthalenesulfonic Acid.

Benzoic Acid is an organic acid with antimicrobial properties.  It is typically used in cosmetics, food, and pharmaceuticals as a preservative against yeast and mold. As treatment, it is also used as a topical medicine of fungal skin infections. It can be naturally found in many berries and vegetable resins. With it’s chemical formula being C7H6O2, it is considered the simplest aromatic carboxylic acid. You can find detailed UV spectra of Benzoic Acid and information about its various lambda maxima by visiting the following link.

2-Naphthalenesulfonic Acid is an organic compound with the formula C10H7SO3H. It is used primarily in the production of dyes, but has also historically been industrially used for a it’s various chemical reactions.

Newcrom BH column is used for HPLC separation of weak and strong organic acids using a mixed-mode method.  Benzoic and naphthalenesulfonic acids are retained based on polar interaction mode and anion-exchange mode.  The isocratic separation is achieved very quickly on a short 3.2 x 50 mm column in under 3 minutes.  The mobile phase is acetonitrile (ACN) and water with sulfuric acid buffer.  UV detection at 275nm.

Condition

Column Newcrom BH, 3.2 x 50 mm, 3 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 80/20%
Buffer H2SO4 – 0.2%
Flow Rate 0.5 ml/min
Detection UV 275nm

Description

Class of Compounds Acid
Analyzing Compounds Benzoic Acid, 2-Naphthalenesulfonic Acid

Application Column

Newcrom BH

Column Diameter: 3.2 mm
Column Length: 50 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
2-Naphthalenesulfonic Acid
Benzoic Acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.