HPLC Method for Analysis of trans-Zeatin on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) MS Method for Analysis of trans-Zeatin on Primesep 100 by SIELC Technologies

Separation type:Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of trans-Zeatin on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of trans-Zeatin

trans-Zeatin is a type of cytokinin, a class of plant hormones that promote cell division and growth, delay aging, and play a crucial role in plant development. It is one of the most active and common forms of zeatin found in plants.

It is often involved in various physiological processes, including seed germination, leaf formation, and root and shoot differentiation.

trans-Zeatin can be retained, separated and analyzed using a Primesep 100 column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN) and Sulfuric acid as a buffer. This method allows for detection using Vis 200 nm.

You can find detailed UV spectra of trans-Zeatin and information about its various lambda maxima by visiting the following link.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O- 20/80%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 202 nm
Sample1 mg/ml
SolventMeCN/H2O- 20/80%
LOD50 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Cytokinin, Plant hormones
Analyzing Compoundstrans-Zeatin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
trans-Zeatin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.