HPLC Method for Analysis of Trimethylglycine (Betaine) on Primesep 100 by SIELC Technologies
Trimethylglycine, also known as betaine, is a compound derived from the amino acid glycine with the chemical formula C5H11NO2. It is found in various foods, including beets, spinach, whole grains, and seafood. Trimethylglycine has been studied for its potential health benefits, and it is commonly used as a dietary supplement. It plays a role in a process called methylation, which is important for various biochemical reactions in the body. Some studies suggest that trimethylglycine may have potential benefits for heart health as it is believed to help lower levels of homocysteine, an amino acid linked to an increased risk of heart disease when present in high concentrations. You can find detailed UV spectra of Trimethylglycine and information about its various lambda maxima by visiting the following link.
Trimethylglycine (Betaine) can be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using UV at 190 nm and SIM 118+
| Column | Primesep 100, 2.1 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | Ammonium formate pH 3.0 – 2 mM |
| Flow Rate | 0.2 ml/min |
| Detection | UV 190 nm, SIM 188+ |
| Samples | 1 mg/mL |
| Injection volume | 1 µl |
| LOD* | 1 ppm |
| Class of Compounds | Quaternary ammonium |
| Analyzing Compounds | Trimethylglycine (Betaine) |
Application Column
Primesep 100
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
