HPLC Method for Analysis of Ethyl Acetate on Newcrom R1 Column

High Performance Liquid Chromatography (HPLC) MS Method for Analysis of Ethyl acetate on Newcrom R1 by SIELC Technologies

Separation type:Liquid Chromatography Reverse Phase SIELC Technologies

HPLC Method for Analysis of Ethyl acetate on Newcrom R1 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethyl acetate

Ethyl acetate (C₄H₈O₂) is a chemical compound classified as an ester. It is produced by the reaction of ethanol and acetic acid (known as esterification). Ethyl acetate is commonly used as a solvent in various applications such as chromatography, paints, coatings, adhesives, and in the production of synthetic fruit essences and flavors.

It is widely utilized in analytical chemistry and extraction processes due to its good solvency for a variety of compounds.

Ethyl acetate can be retained, separated and analyzed using a Newcrom R1 column. The analysis employs an isocratic method with a simple mobile phase comprising water and acetonitrile (MeCN). This method allows for detection using Vis 200 nm.

You can find detailed UV spectra of Ethyl acetate and information about its various lambda maxima by visiting the following link.

ColumnNewcrom R1, 3.2 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O- 20/80%
BufferNone
Flow Rate0.5 ml/min
DetectionUV 206 nm
Sample1 mg/ml
DiluentMeCN/H2O- 20/80%
LOD0.4 ppm
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Ester
Analyzing CompoundsEthyl acetate

Application Column

Newcrom R1

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Ethyl acetate

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.