High Performance Liquid Chromatography (HPLC) Method for Analysis of Sertraline on Primesep B by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sertraline
Sertraline is an antidepressant belonging to the class of selective serotonin reuptake inhibitors (SSRIs). It is commonly prescribed to treat conditions like depression, anxiety disorders, obsessive-compulsive disorder (OCD), panic attacks, and post-traumatic stress disorder (PTSD).
Mechanism of Action
Sertraline works by increasing the levels of serotonin, a neurotransmitter that plays a key role in mood regulation, by inhibiting its reuptake in the brain. This leads to improved mood, reduced anxiety, and better overall mental health for individuals with serotonin imbalances.
Common Uses
Major depressive disorder (MDD)
Generalized anxiety disorder (GAD)
Panic disorder
Obsessive-compulsive disorder (OCD)
Post-traumatic stress disorder (PTSD)
Social anxiety disorder
Sertraline can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 195 nm.
You can find detailed UV spectra of Sertraline and information about its various lambda maxima by visiting the following link.
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 195 nm |
| Limit of Detection | 4 ppb |
| Class of Compounds | Drug |
| Analyzing Compounds | Sertraline |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
