| CAS Number | 79559-97-0 |
|---|---|
| Molecular Formula | C17H18Cl3N |
| Molecular Weight | 342.691 |
| InChI Key | BLFQGGGGFNSJKA-XHXSRVRCSA-N |
| LogP | 4.73 |
| Synonyms |
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Applications:
UV-Vis Spectrum of Sertraline
September 4, 2024
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Sertraline in Tablet Dosage on Primesep B Column
September 4, 2024
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sertraline on Primesep B by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sertraline
Sertraline is an antidepressant belonging to the class of selective serotonin reuptake inhibitors (SSRIs). It is commonly prescribed to treat conditions like depression, anxiety disorders, obsessive-compulsive disorder (OCD), panic attacks, and post-traumatic stress disorder (PTSD).
Mechanism of Action
Sertraline works by increasing the levels of serotonin, a neurotransmitter that plays a key role in mood regulation, by inhibiting its reuptake in the brain. This leads to improved mood, reduced anxiety, and better overall mental health for individuals with serotonin imbalances.
Common Uses
Major depressive disorder (MDD)
Generalized anxiety disorder (GAD)
Panic disorder
Obsessive-compulsive disorder (OCD)
Post-traumatic stress disorder (PTSD)
Social anxiety disorder
Sertraline can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 195 nm.
You can find detailed UV spectra of Sertraline and information about its various lambda maxima by visiting the following link.
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 195 nm |
| Limit of Detection | 4 ppb |
| Class of Compounds | Drug |
| Analyzing Compounds | Sertraline |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Separation of Sertraline hydrochloride on Newcrom R1 HPLC column
February 16, 2018
Sertraline hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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