High Performance Liquid Chromatography (HPLC) Method for Analysis of Isoproturon on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Isoproturon
Isoproturon is classified as a phenylurea herbicide. It is used primarily for controlling annual grasses and broadleaf weeds in cereal crops such as wheat and barley. Isoproturon works by inhibiting photosynthesis in the targeted plants, which eventually leads to their death. It is absorbed by the roots and shoots of the plants and is most effective when applied before or during the early stages of weed growth.
Isoproturon can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm.
You can find detailed UV spectra of Isoproturon and information about its various lambda maxima by visiting the following link.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 45% |
| Buffer | H2SO4 -0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Samples | 1.0 mg/ml in MeCN – 100% |
| Injection volume | 1 µl |
| LOD* | 10 ppb (250 nm) |
| Class of Compounds | phenylurea herbicide |
| Analyzing Compounds | Isoproturon |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
