HPLC Method for Tofisopam on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tofisopam
Tofisopam is an anxiolytic medication belonging to the benzodiazepine class, but it differs from traditional benzodiazepines in that it has no significant sedative, muscle relaxant, or anticonvulsant properties. It is mainly used to treat anxiety and stress-related disorders while allowing the user to remain alert and functional.
Unlike classic benzodiazepines like diazepam or alprazolam, tofisopam does not cause drowsiness and has a lower potential for addiction and dependence. It is commonly prescribed in some countries, particularly in Eastern Europe and parts of Asia, but is not widely available in the U.S. or many Western countries.
Tofisopam can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an gradientc method with a simple mobile phase consisting of water, acetonitrile (MeCN), and HClO4. Detection is performed using UV at 205nm.
| Column | Newcrom R1, 2.1 x 100 mm, 3 µm, 100 A |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | HClO4 – 0.1% |
| Flow Rate | 0.2 ml/min |
| Detection | UV 205 nm |
| LOD | 1 ppb |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | Drug |
| Analyzing Compounds | Tofisopam |
Application Column
Newcrom R1
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
