Tofisopam

Tofisopam structural formula

CAS Number22345-47-7
Molecular FormulaC22H26N2O4
Molecular Weight382.460
InChI KeyRUJBDQSFYCKFAA-UHFFFAOYSA-N
LogP3.57
Synonyms
  • Tofisopam
  • 1-(3,4-Dimethoxyphenyl)-5-ethyl-7,8-dimethoxy-4-methyl-5H-2,3-benzodiazepine
  • 5H-2,3-Benzodiazepine, 1-(3,4-dimethoxyphenyl)-5-ethyl-7,8-dimethoxy-4-methyl-
  • 22345-47-7
  • EINECS 244-922-3
  • Grandaxin
  • Tofisopamum
  • UNII-UZC80HAU42

Applications:

HPLC-MS Method for the Analysis of Tofisopam in Pharmaceutical Formulation on Newcrom R1 Column

March 5, 2025

HPLC Method for Tofisopam on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tofisopam


High Performance Liquid Chromatography (HPLC) Method for Analysis of Tofisopam

Tofisopam is an anxiolytic medication belonging to the benzodiazepine class, but it differs from traditional benzodiazepines in that it has no significant sedative, muscle relaxant, or anticonvulsant properties. It is mainly used to treat anxiety and stress-related disorders while allowing the user to remain alert and functional.

Unlike classic benzodiazepines like diazepam or alprazolam, tofisopam does not cause drowsiness and has a lower potential for addiction and dependence. It is commonly prescribed in some countries, particularly in Eastern Europe and parts of Asia, but is not widely available in the U.S. or many Western countries.

Tofisopam can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an gradientc method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid. Mass spectrometric detection was carried out in ESI-SIM mode, monitoring the ions [M+H]⁺ at m/z 383

ColumnNewcrom R1, 3.2 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 20-80%, 10 min
BufferFormic acid – 2%
Flow Rate0.2 ml/min
DetectionESI-SIM: [M+H]1⁺  383
LOD0.1 ppb

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsDrug
Analyzing CompoundsTofisopam

Application Column

Newcrom R1

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Tofisopam

Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

UV-Vis Spectrum of Tofisopam

March 3, 2025
UV-Vis Spectrum of Keracyanin. Absorption Maxima: 204 nm, 253 nm, 350 nm.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Tofisopam
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for the Analysis of Tofisopam in Pharmaceutical Formulation on Newcrom R1 Column

February 28, 2025

HPLC Method for Tofisopam on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tofisopam


High Performance Liquid Chromatography (HPLC) Method for Analysis of Tofisopam

Tofisopam is an anxiolytic medication belonging to the benzodiazepine class, but it differs from traditional benzodiazepines in that it has no significant sedative, muscle relaxant, or anticonvulsant properties. It is mainly used to treat anxiety and stress-related disorders while allowing the user to remain alert and functional.

Unlike classic benzodiazepines like diazepam or alprazolam, tofisopam does not cause drowsiness and has a lower potential for addiction and dependence. It is commonly prescribed in some countries, particularly in Eastern Europe and parts of Asia, but is not widely available in the U.S. or many Western countries.

Tofisopam can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an gradientc method with a simple mobile phase consisting of water, acetonitrile (MeCN), and HClO4. Detection is performed using UV at 205nm.

ColumnNewcrom R1, 2.1 x 100 mm, 3 µm, 100 A
Mobile PhaseMeCN/H2O – 40/60%
BufferHClO4 – 0.1%
Flow Rate0.2 ml/min
DetectionUV 205 nm
LOD1 ppb

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsDrug
Analyzing CompoundsTofisopam

Application Column

Newcrom R1

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Tofisopam

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Tofisopam on Newcrom R1 HPLC column

February 16, 2018
Separation of Tofisopam on Newcrom C18 HPLC column

Tofisopam can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Tofisopam
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.