| CAS Number | 22345-47-7 |
|---|---|
| Molecular Formula | C22H26N2O4 |
| Molecular Weight | 382.460 |
| InChI Key | RUJBDQSFYCKFAA-UHFFFAOYSA-N |
| LogP | 3.57 |
| Synonyms |
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Applications:
HPLC-MS Method for the Analysis of Tofisopam in Pharmaceutical Formulation on Newcrom R1 Column
March 5, 2025
HPLC Method for Tofisopam on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tofisopam
Tofisopam is an anxiolytic medication belonging to the benzodiazepine class, but it differs from traditional benzodiazepines in that it has no significant sedative, muscle relaxant, or anticonvulsant properties. It is mainly used to treat anxiety and stress-related disorders while allowing the user to remain alert and functional.
Unlike classic benzodiazepines like diazepam or alprazolam, tofisopam does not cause drowsiness and has a lower potential for addiction and dependence. It is commonly prescribed in some countries, particularly in Eastern Europe and parts of Asia, but is not widely available in the U.S. or many Western countries.
Tofisopam can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an gradientc method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid. Mass spectrometric detection was carried out in ESI-SIM mode, monitoring the ions [M+H]⁺ at m/z 383
| Column | Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeCN – 20-80%, 10 min |
| Buffer | Formic acid – 2% |
| Flow Rate | 0.2 ml/min |
| Detection | ESI-SIM: [M+H]1⁺ 383 |
| LOD | 0.1 ppb |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | Drug |
| Analyzing Compounds | Tofisopam |
Application Column
Newcrom R1
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
UV-Vis Spectrum of Tofisopam
March 3, 2025
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for the Analysis of Tofisopam on Newcrom R1 Column
February 28, 2025
HPLC Method for Tofisopam in Pharmaceutical Formulation on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tofisopam
Tofisopam is an anxiolytic medication belonging to the benzodiazepine class, but it differs from traditional benzodiazepines in that it has no significant sedative, muscle relaxant, or anticonvulsant properties. It is mainly used to treat anxiety and stress-related disorders while allowing the user to remain alert and functional.
Unlike classic benzodiazepines like diazepam or alprazolam, tofisopam does not cause drowsiness and has a lower potential for addiction and dependence. It is commonly prescribed in some countries, particularly in Eastern Europe and parts of Asia, but is not widely available in the U.S. or many Western countries.
Tofisopam can be retained and analyzed using the Newcrom R1 column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and HClO4. Detection is performed using UV at 205nm.
| Column | Newcrom R1, 2.1 x 100 mm, 3 µm, 100 A |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | HClO4 – 0.1% |
| Flow Rate | 0.2 ml/min |
| Detection | UV 205 nm |
| LOD | 1 ppb |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | Drug |
| Analyzing Compounds | Tofisopam |
Application Column
Newcrom R1
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Separation of Tofisopam on Newcrom R1 HPLC column
February 16, 2018
Tofisopam can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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