HPLC MS Method for Analysis of Phytic Acid on BIST A Column

HPLC Method for Phytic Acid on BIST A by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Phytic Acid

Phytic Acid, also known as inositol hexaphosphate, inositol hexakisphosphate (IP6), AND inositol polyphosphate is a dihydrogenphosphate ester of inositol with the molecular formula C6H18O24P6. It is also known as a phosphorus-storage compounds primarily found in nuts, seeds, and whole grains. It serves as a way for plants to store phosphorus. When broken down in the body, it binds to chelates.

Phytic Acid can be retained and analyzed using the BIST A stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.

ColumnBIST A, 2.1 x 50 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient MeCN – 60/40% – 10/90%, 10 min
BufferTMEDA formate
Flow Rate0.2 mL/min
DetectionLC MS ESI SIM
LOD* UV2 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Class of CompoundsEster
Analyzing CompoundsPhytic Acid

Application Column

BIST A

Column Diameter: 2.1 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Phytic Acid
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.