HPLC Method for Analysis of Cyanamide, Dicyandiamide, Urea on Primesep S Column by SIELC Technologies

Cyanamide is a highly reactive and toxic compound with the chemical formula H₂CN₂. It is primarily used in the production of fertilizers, pharmaceuticals, and other chemicals. It can be used as a soil fumigant to control pests and weeds. Cyanamide became known the first known interstellar molecule to contain the NCN when it was detected in spectral emissions from the Sgr B2 molecular cloud.

Dicyandiamide is a white crystalline solid with the chemical formula C₂H₄N₄. It is often used as a slow-release fertilizer. It is also used in the production of pharmaceuticals, dyes, and resins. Dicyandiamide can also be used as a nitrification inhibitor in agriculture to reduce the loss of nitrogen from the soil. You can find detailed UV spectra of Dicyandiamide and information about its various lambda maxima by visiting the following link.

Urea is a white crystalline solid with the chemical formula CO(NH₂)₂. It has a wide variety of uses including but not limited to as fertilizer, due to its high nitrogen content, In the production of plastics, resins, and adhesives, in the manufacturing of animal feed, and as a raw material in the production of many industrial chemicals. In addition, it is also used as a component in certain skin creams and cosmetics due to its moisturizing properties. You can find detailed UV spectra of Urea and information about its various lambda maxima by visiting the following link.

Using a Primesep S normal-phase column and a mobile phase consisting of water and Acetonitrile (MeCN) with no buffer, Cyanamide, Dicyandiamide and Urea can be retained, separated, and analyzed. This analysis method can be UV detected at 200 nm.

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