Coumarin is an aromatic chemical that is used as a fragrance ingredient. It has a sweet earthy scent and is typically found in Tonka beans, cinnamon, woodruff, bison grass, and green tea. Besides a pleasant smell it had anti-inflammatory, antibacterial, and antifungal properties.
Coumarin an be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
| Column | Newcrom R1 3.2×100 mm, 3 µm, 100A |
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | H3PO4 – 0.1 % |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 276 nm |
| Class of Compounds | Drug, Flavouring substance, Hydrophobic, Neutral |
| Analyzing Compounds | Coumarin |
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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