HPLC Method for Analysis of Estetrol, Estiol, Estrone, and Besta-Estradiol on Chromni Column

HPLC Method for Estrone, Estradiol, Estetrol, Estriol on Chromni™ by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Estrone, Estradiol, Estetrol, Estriol

Estetrol is an synthetic organochlorine compound with the molecular formula C18H24O4.

Properties:
Appearance: Typically a white, odorless, powder.

Molecular weight: ~270.4 g/mol

Solubility: Soluble in water.

Uses: Production of contraceptives.

Estriol is an synthetic organochlorine compound with the molecular formula C18H24O3.

Properties:
Appearance: Typically a white, odorless, powder.

Molecular weight: ~288.4 g/mol

Solubility: Soluble in benzene, water, alcohol, and vegetable oils.

Uses: Hormone Replacement Therapy and similar medication.

Estrone is an synthetic organochlorine compound with the molecular formula C18H22O2.

Properties:
Appearance: Typically a white, odorless, crystals.

Molecular weight: ~270.4 g/mol

Solubility: Soluble in benzene, water, acetone, and vegetable oils.

Uses: Production of medication

Beta-Estradiol is an synthetic organochlorine compound with the molecular formula C18H24O2.

Properties:
Appearance: Typically a white, odorless, crystals.

Molecular weight: ~272.4 g/mol

Solubility: Soluble in ethanol, water, and vegetable oils.

Uses: Production of medication for treatment of menopausal symptoms

Estrone, Estradiol, Estetrol, Estriol can be retained and analyzed using the Chromni™ stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate. Detection is performed using UV.

ColumnChromni™, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN – 20%
BufferAmmonium Formate – 10mM
Flow Rate1.0 ml/min
DetectionUV 280 nm

Class of CompoundsEstrogen
Analyzing CompoundsEstrone, Estradiol, Estetrol, Estriol

Application Column

Chromni™

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Estetrol
Estradiol
Estriol
Estrone
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.