HPLC Method for Separation of Rutin and Kaempferol on Newcrom R1 Column

HPLC Method for Analysis of Rutin, Kaempferol on Newcrom R1 Column by SIELC Technologies

Separation type: Liquid Chromatography Reversed-phase

HPLC Method for Separation of Rutin and Kaempferol on Newcrom R1 Column
HPLC Method for Analysis of Rutin, Kaempferol on Newcrom R1 Column by SIELC Technologies

Rutin and kaempferol are both natural compounds found in various plants, particularly in fruits and vegetables. They belong to the class of flavonoids, which are a group of polyphenolic compounds known for their antioxidant properties and potential health benefits.

  1. Rutin:
  • Chemical Structure: Rutin, also known as rutoside, quercetin-3-O-rutinoside, or vitamin P, is a glycoside of the flavonoid quercetin. It consists of quercetin bound to the disaccharide rutinose.
  • Sources: Rutin is found in various foods, including citrus fruits, apples, berries, buckwheat, and green tea.
  • Health Benefits: Rutin is known for its antioxidant properties and has been studied for potential benefits, such as supporting cardiovascular health, reducing inflammation, and promoting blood vessel health.
  1. Kaempferol:
  • Chemical Structure: Kaempferol is a flavonol, a subtype of flavonoids. Its chemical structure includes a flavone backbone with hydroxyl groups.
  • Sources: Kaempferol is present in various fruits and vegetables, such as apples, berries, tomatoes, broccoli, tea, and onions.
  • Health Benefits: Kaempferol is recognized for its antioxidant and anti-inflammatory properties. It has been studied for potential effects on reducing the risk of chronic diseases, including cardiovascular diseases and certain types of cancer. Additionally, it may have neuroprotective properties.

Both rutin and kaempferol are considered bioactive compounds, and their presence in a balanced diet rich in fruits and vegetables is associated with potential health benefits.

Rutin and kaempferolcan be retained, separated and analyzed on a reversed-phase Newcrom R1 column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid. This analytical method can be detected with high resolution and peak symmetry at a wavelength of 265 nm using UV detection

High Performance Liquid Chromatography (HPLC) Method for Analyses of Rutin, Kaempferol on Newcrom R1 Column by SIELC Technologies

Condition

ColumnNewcrom R1, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 10-90%,10 min , 5 min hold
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 265 nm
Injection volume:   5.0 µL
Sample1. 0.5 mg/ml
2. 0.5 mg/ml
Sample DiluentMeCN/H2O – 50/50%
LOD*  1. 9 ppb
  2. 14 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of Compoundsflavonoids
Analyzing CompoundsRutin, Kaempferol

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Kaempferol
Rutin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.