HPLC Method for Determination of Ketoconazole in Nizoral Shampoo on Primesep B by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Ketoconazole, also known as Nizoral, is an antifungal compound used to treat dandruff and is commonly available as a shampoo. Ketoconazole can be retained, analyzed, and separated from Benzyl alcohol on a Primesep B reverse-phase column using a gradient analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Ammonium formate (AmFm) as the ionic modifier. This analysis method can be UV detected at 250 nm with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ketoconazole
Condition
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeCN – 10-50% |
| Buffer | Ammonium Formate pH 3.5-20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Peak Retention Time | 6.72 |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Ketoconazole |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
Ketoconazole
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
