High Performance Liquid Chromatography (HPLC) Method for Analysis of Polyhexanide PHMB, Alexidine on BIST B+ by SIELC Technologies
Alexidine is a chemical compound that belongs to the class of bis-biguanide antiseptics and has the chemical formula C26H56N10. It is known for its antimicrobial properties and is used in various products for disinfection and antiseptic purposes. Alexidine has broad-spectrum antimicrobial activity, and it is commonly used in healthcare settings for its effectiveness against bacteria, fungi, and some viruses. In some formulations, alexidine is used as a topical antiseptic to disinfect skin and mucous membranes. It is found in some mouthwashes, oral care products, contact lens solutions, and more It may be included in certain wound care products for its ability to prevent infection. You can find detailed UV spectra of Alexidine and information about its various lambda maxima by visiting the following link.
Polyhexanide (polyhexamethylene biguanide, PHMB) is a disinfectant with the chemical formula (C8H17N5)n. It has multiple industrial, medical, biological and research applications especially for materials that could be a possible source of bacterial contamination. Dermatologically, it is sold as polihexanide. From 2011, it has been classified as a category 2 carcinogen. You can find detailed UV spectra of Polyhexanide PHMB and information about its various lambda maxima by visiting the following link.
Polyhexanide PHMB, Alexidine can be retained, separated and analyzed on a BIST B+ mixed-mode stationary phase column using an analytical method with a simple mobile phase of water, Acetonitrile (MeCN) , and a sulfuric acid as a buffer. This analysis method can be detected using UV at 200 nm.
Condition
| Column | BIST B+, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase* | Step Gradient A: MeCN – 75%, H2SO4 – 0.1% / 3 min B: MeCN – 0%, H2SO4 – 0.1% / 6 min |
| Buffe | H2SO4 -0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Sample | 1. 0.14 mg/ml 2. 0.26 mg/ml |
| LOD ** | 8 ppb, 10 ppb |
| Injection volume | 10 µl |
*The step gradient methods involve sudden changes in the solvent composition of the mobile phase, resulting in the baseline’s instability. It is necessary to account for this response to be able to interpret, integrate, and process results adequately. The blank sample (sample diluent) has to be analyzed before injecting the sample of interest. When all chromatograms are generated, it is necessary to subtract the blank chromatogram from the sample one before the integration step.
**LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed
Description
| Class of Compounds | Bis-biguanide |
| Analyzing Compounds | Polyhexanide PHMB, Alexidine |
Application Column
BIST B+
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Polyhexanide PHMB
