| CAS Number | 51276-47-2 |
|---|---|
| Molecular Formula | C5H12NO4P |
| Molecular Weight | 181.3 |
| InChI Key | ZBMRKNMTMPPMMK-UHFFFAOYSA-N |
| LogP | -5 |
| Synonyms |
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Applications:
UV-Vis Spectrum of Glufosinate
December 2, 2025
If you are looking for optimized HPLC method to analyze Glufosinate check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Separation of Glufosinate, Glyphosate and Glyphosine on Newcrom B Column
December 10, 2020
HPLC Method for Glufosinate, Glyphosate, Glyphosine on Newcrom B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Glufosinate, Glyphosate, Glyphosine.
Glufosinate is a naturally occurring herbicide. It is produced by Streptomyces soil bacteria and is used for weed control against broadleaf and grass weeds. It’s chemical formula is C5H12NO4P. The herbicide works through inhibiting glutamine synthetase, which is a key enzyme in plant metabolism. It is a sky and eye irritant, therefore it is encouraged to wear protective gear when handling Glufosinate.
Glyphosate is a broad-spectrum herbicide. It works through inhibiting the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase. The enzyme is essential for producing amino acids within the plant. Glyphosate is used across agriculture and forestry, as well as rare aquatic environments. It’s chemical formula is C3H8NO5P.
Glyphosine is a synthetic plant growth regulator. It is primarily used to hasten maturity of sugarcane., but at high levels it can also be used as herbicide due to it’s ability to cause chlorosis in emerging leaves. It’s chemical formula is C4H11NO8P2.
Glufosinate, Glyphosate, Glyphosine can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | Formic Acid Gradient |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Hydrophobic, Herbicide, Pesticide |
| Analyzing Compounds | Glufosinate, Glyphosate, Glyphosine |
Application Column
Newcrom B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Glyphosate
Glyphosine
Separation of Glufosinate-ammonium on Newcrom R1 HPLC column
February 16, 2018
Glufosinate-ammonium can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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