Separation Study of Zemuron Active Ingredient in Mixed-Mode HPLC
May 5, 2010
Zemuron is an amino steroid used as a muscle relaxant during anesthesia. Zemuron was analyzed by mixed-mode chromatography on a Primesep C HPLC column. Compound is hydrophobic and basic in nature, and retention time on cation-exchange mixed-mode column is controlled by the amount of acetonitrile, buffer concentration and buffer pH. In order to observe cation-exchange interaction on a Primesep C column, mobile phase pH should be maintained between 3-7.
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.Select options