Separation Study of Zemuron Active Ingredient in Mixed-Mode HPLC

Zemuron is an amino steroid used as a muscle relaxant during anesthesia. Zemuron was analyzed by mixed-mode chromatography on a Primesep C HPLC column. Compound is hydrophobic and basic in nature, and retention time on cation-exchange mixed-mode column is controlled by the amount of acetonitrile, buffer concentration and buffer pH. In order to observe cation-exchange interaction on a Primesep C column, mobile phase pH should be maintained between 3-7.

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.