4-Aminopyridine/Fampridine structural formula

CAS Number504-24-5
Molecular FormulaC5H6N2
Molecular Weight94.117
  • 4-Aminopyridine
  • Pyridin-4-amine
  • 4-Pyridinamine
  • 504-24-5
  • 5-22-09-00106
  • 4-Pyridinamine
  • 4-Amino-Pyridine
  • 4-piridilamina
  • 4-Pyridinylamine
  • 4-Pyridylamin
  • 4-Pyridylamine
  • Avitrol
  • Fampridine
  • Neurelan
  • NSC 15041
  • p-Aminopyridine
  • Phillips 1861
  • Pyridine-4-ylamine
  • γ-Aminopyridine
  • Amino-4 pyridine
  • Avitrol 200
  • BRN 0105782
  • Caswell No. 038
  • Compound 1861
  • EINECS 207-987-9
  • EPA Pesticide Chemical Code 069201
  • 4-AP
  • Dalfampridine
  • N07XX07
  • gamma-Aminopyridine


HPLC Separation of Vitamin C, Vitamin Group B, and Related Impurities

August 22, 2008

Vitamin C (ascorbic acid) and Vitamins Group B are separated on Obelisc N mixed-mode column. Method can be used in quantitation and determination of polar vitamins in various formulations and dietary supplements. HPLC method can be based on UV, Evaporative Light Scattering Detection (ESLD), RI or MS detection. Effect of sample matrix can be eliminated by changing mobile phase conditions. Buffer concentration, buffer pH and amount of ACN will affect every vitamin differently due to difference in polar and ionic properties.


Column Obelisc N , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 5.0
Flow Rate 1.0 ml/min
Detection UV, 250 nm



Class of Compounds
Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Pyridoxine, Ascorbic acid, Niacinamide, Pantothenic acid, Caffeine, Riboflavin, Barbituric Acid,  3- Aminopyrine

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:

Ascorbic Acid
Barbituric Acid
Isonicotinic Acid
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.