C.I. Direct Blue 53

C.I. Direct Blue 53 structural formula

CAS Number314-13-6
Molecular FormulaC34H24N6Na4O14S4
Molecular Weight960.790
InChI KeyATNOAWAQFYGAOY-GPTZEZBUSA-J
LogP2.58
Synonyms
  • C.I. Direct Blue 53
  • Tetrasodium 6,6'-{(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis[(E)diazene-2,1-diyl]}bis(4-amino-5-hydroxynaphthalene-1,3-disulfonate)
  • 1,3-Naphthalenedisulfonic acid, 6,6'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)di(E)-2,1-diazenediyl]bis[4-amino-5-hydroxy-, sodium salt (1:4)
  • 314-13-6
  • Evans blue
  • C.I. direct blue 53 tetrasodium salt
  • Azovan Blue
  • 4,4'-Bis(1-amino-8-hydroxy-2,4-disulfo-7-naphthylazo)-3,3'-bitolyl, tetrasodium salt
  • 4,4'-Bis(7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo)-3,3'-bitolyl, tetrasodium salt
  • Diamine Sky Blue FF
  • Diazobleu
  • Diazol Pure Blue BF
  • Direct Blue 53
  • Dye Evans Blue
  • EINECS 206-242-5
  • Evablin
  • Evans Blue Dye
  • Evans blue, sodium salt
  • Geigy Blue 536, med
  • Geigy-blau 536
  • NSC 8680
  • Blekit evansa
  • Modr Evansova
  • Modr Prima 53
  • UNII-45PG892GO1
  • 4,4'-Bis(1-amino-8-hydroxy-2,4-disulpho-7-naphthylazo)-3,3'-bitolyl, tetrasodium salt
  • 4,4'-Bis(7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo)-3,3'-bitolyl, tetrasodium salt
  • 4,4'-Bis(7-(1-amino-8-hydroxy-2,4-disulpho)naphthylazo)-3,3'-bitolyl, tetrasodium salt
  • C.I. direct blue 53 tetrasodium salt
  • 179472-53-8

Applications:

Separation of C.I. Direct Blue 53 on Newcrom R1 HPLC column

May 16, 2018
Separation of C.I. Direct Blue 53 on Newcrom R1 HPLC column

C.I. Direct Blue 53 can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
C.I. Direct Blue 53
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.