Bilirubin structural formula

CAS Number635-65-4
Molecular FormulaC33H36N4O6
Molecular Weight584.673
  • Bilirubin
  • 635-65-4
  • 5-26-15-00523
  • 21H-Biline-8,12-dipropanoic acid, 2,17-diethenyl-1,10,19,22,23,24-hexahydro-3,7,13,18-tetramethyl-1,19-dioxo-
  • (4Z,15Z)-Bilirubin IX?
  • (Z,Z)-Bilirubin
  • (Z,Z)-Bilirubin IX?
  • Biline-8,12-dipropionic acid, 1,10,19,22,23,24-hexahydro-2,7,13,17-tetramethyl-1,19-dioxo-3,18-divinyl-
  • Bilirubin IX?
  • bilirubina
  • Bilirubine
  • Cholerythrin
  • Hematoidin
  • NSC 26685
  • Bilirubin IX-alpha
  • BRN 0074376
  • EINECS 211-239-7
  • Hemetoidin
  • Principal bile pigment
  • 11053-42-2
  • 114-24-9
  • 19245-52-4
  • 39372-61-7
  • 493-86-7
  • 55527-40-7
  • 856927-39-4
  • 917-01-1


Separation of Bilirubin on Newcrom R1 HPLC column

May 16, 2018
Separation of Bilirubin on Newcrom R1 HPLC column

Bilirubin is a compound that the body produces naturally through the breakdown of red blood cells. It makes urine yellow. Bilirubin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: or by phone: 847-229-2629.