| CAS Number | 132-17-2 |
|---|---|
| Molecular Formula | C22H29NO4S |
| Molecular Weight | 403.541 |
| InChI Key | CPFJLLXFNPCTDW-BWSPSPBFSA-N |
| LogP | 4.18 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Benztropine Mesylate
March 13, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Benztropine methylsulfonate check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Benztropine mesylate on Primesep SB Column
April 29, 2019
HPLC Method for Benztropine methylsulfonate on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Benztropine methylsulfonate.
Benztropine is a medical drug used in treatment of patients with Parkinson’s disease. It works as an acetylcholine blocker decreasing muscle stiffness. Benztropine can be retained in HPLC on Primesep SB mixed-mode column by hydrophobic and anion-exchange properties. The isocratic method uses a mobile phase of acetonitrile (ACN) and water with sulfuric acid buffer and UV detection at 220nm.
| Column | Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 25/75% |
| Buffer | H2SO4 – 0.1 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 220 nm |
| Class of Compounds |
Drug, Basic, Hydrophobic, Ionizable, Zwitterionic |
| Analyzing Compounds | Benztropine methylsulfonate |
Application Column
Primesep SB
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Separation of Benztropine methylsulfonate on Newcrom R1 HPLC column
February 16, 2018
Benztropine methylsulfonate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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