Cotinine Metabolites

Cotinine Metabolites

Synonyms

    Applications:

    HPLC Separation of Cotinine Metabolites

    June 29, 2004

    Primesep 200 separates the very hydrophilic cotinine metabolites. Ion-exchange and other polar interactions provide retention for a compound that is difficult to retain on traditional C18 reversed-phase columns. The separation uses a simple mobile phase of water, acetonitrile (MeCN, ACN), and trifluoracetic acid (TFA) with UV detection.

    Condition
    Column Primesep 200, 3.0×50 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer TFA
    Flow Rate 1.0 ml/min
    Detection UV, 23, 250, 270 nm

     

    Description
    Class of Compounds
    		 Hydrophilic
    Analyzing Compounds Cotinine Metabolites

     

    Application Column

    Primesep 200

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    Cotinine
    Cotinine Metabolites
    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.