HPLC Separation of Cotinine Metabolites

Primesep 200 separates the very hydrophilic cotinine metabolites. Ion-exchange and other polar interactions provide retention for a compound that is difficult to retain on traditional C18 reversed-phase columns. The separation uses a simple mobile phase of water, acetonitrile (MeCN, ACN), and trifluoracetic acid (TFA) with UV detection.


Column Primesep 200, 3.0x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV, 23, 250, 270 nm


Class of Compounds Hydrophilic
Analyzing Compounds Cotinine Metabolites
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Application Analytes:
Cotinine Metabolites

Application Detection:
UV Detection