| CAS Number | 14432-12-3 |
|---|---|
| Molecular Formula | C5H5ClN2 |
| Molecular Weight | 128.559 |
| InChI Key | BLBDTBCGPHPIJK-UHFFFAOYSA-N |
| LogP | 1.1 |
| Synonyms |
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Applications:
HPLC Method for Analysis of 4-Amino-2-chloropyridine on Primesep 100 Column
July 30, 2024
High Performance Liquid Chromatography (HPLC) Method for Analysis of 4-Amino-2-Chloropyridine on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of 4-Amino-2-Chloropyridine
4-Amino-2-chloropyridine is a chemical compound with the molecular formula C5H5ClN2. It’s a derivative of pyridine, featuring both an amino group (-NH2) and a chlorine atom as substituents.
4-Amino-2-chloropyridine is often used in organic synthesis and pharmaceuticals. It can serve as an intermediate in the production of various active pharmaceutical ingredients (APIs) and in the synthesis of other complex organic molecules.
4-Amino-2-Chloropyridine be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 45% |
| Buffer | H2SO4 -0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Samples | 1.0 mg/ml MeCN/H2O – 50/50% |
| Injection volume | 1 µl |
| LOD* | 8 ppb (200 nm) |
| Class of Compounds | Pyridines |
| Analyzing Compounds | 4-Amino-2-Chloropyridine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Separation of Model Compounds in Reversed-Phase and Mixed-Mode
April 25, 2019
Separation type: Liquid Chromatography Mixed-mode
Many compounds are difficult, if not impossible, to separate on reverse-phase columns in HPLC. Other compounds cannot be separated on ion-exchange columns. That’s where the mixed-mode columns come in. By using a stationary phase with both hydrophobic and ion-exchange properties, allows the chromatographer to have additional controls over separation conditions. Here, we demonstrate the separation of compounds that can’t be achieved on a C18 column. By using both an organic gradient and buffer gradient of ammonium formate (AmFm), we can separate structurally similar compounds that can’t be separated on a reverse-phase column alone.
| Column | Primesep 100, 3,2×50 mm, 2,7 µm, 100A |
| Mobile Phase | Gradient MeCN – 10-60%, 5 min |
| Buffer | Gradient AmFm pH 3.5- 30 – 70 mM, 5 min |
| Flow Rate | 1.2 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Basic, Hydrophilic, Hydrophobic, Ionizable. |
| Analyzing Compounds | Adenosine, 3,4-Difluroaniline, 4-Amino-2-chloropyridine, 5-Aminoindole, 4-Amino-3-chloropyridine, 2-Amino 5-methylthiadiazole, 4-Ethylaniline |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options2-Amino-5-methyl-thiazole
3,4-Difluoroaniline
4-Amino-2-Chloropyridine
4-Amino-3-Chloropyridine
4-Ethylaniline
5-Aminoindole
Adenosine
