TRIS or tris(hydroxymethyl)aminomethane, is a hydrophilic basic compounds widely used in chemistry, biochemistry, and molecular biology. TRIS pKa (8.1) makes it an effective buffer in pH range of 7-9.2. TRIS is not retained on reversed-phase column and is not UV active, making it hard to be analyzed by HPLC. TRIS was analyzed by mixed-mode HPLC on a Primesep 100 column. Retention time of compound is controlled by the amount and pH of the buffer. No ion-pairing reagent is required for HPLC retention of hydrophilic basic molecules like TRIS. TRIS can be monitored by ELSD/CAD with trifluoroacetic acid in the mobile phase, or by LC/MS with ammonium formate or acetate in the mobile phase.
High Performance Liquid Chromatography (HPLC) Method for Analyses of Trometamol (Tris, Tris(hydroxymethyl)aminomethane, Tromethamine, and or THAM)
| Column | Primesep D, Primesep 100 |
| Mobile Phase | MeCN/H2O |
| Buffer | Formic Acid |
| Flow Rate | 0.5 , 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Amine |
| Analyzing Compounds | Tris (Tris(hydroxymethyl)aminomethane, Tromethamine, and or THAM) |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsTrometamol (Tris, Tris(hydroxymethyl)aminomethane, Tromethamine, and or THAM)
