HPLC Separation of Chloride and Nitrate Ions

 

High nitrate concentrations in water and soil can be toxic to humans, fish and domestic animals.  Chloride can be used for analysis of water contamination. Primesep D column can be used to separate and quantify both ions.  The baseline separation between chloride and nitrate can be increased by decreasing the ammonium formate concentration in the mobile phase.  Evaporative Light Scattering Detection (ELSD) used.

Condition
Column Primesep D, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 1.0 ml/min
Detection ELSD

 

Description
Class of Compounds
 Hydrophilic, Ion
Analyzing Compounds Chloride, Nitrate

 

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Chloride
Nitrate
Nitric Acid
Sodium
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.