| CAS Number | 35303-76-5 |
|---|---|
| Molecular Formula | C8H12N2O2S |
| Molecular Weight | 200.260 |
| InChI Key | FXNSVEQMUYPYJS-UHFFFAOYSA-N |
| LogP | -0.376 |
| Synonyms |
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Applications:
HPLC Method for Analysis of 4-(2-Aminoethyl) benzenesulfonamide on Primesep 100 Column
January 23, 2023
HPLC Method for Analysis of 4-(2-Aminoethyl) benzenesulfonamide on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
4-(2-Aminoethyl) benzenesulfonamide, when combined with hypoxic conditions, can inhibit the growth and induce apoptosis of human hepatocellular carcinoma cells. This Benzenesulfonamide can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) buffer. This analysis method can be detected in the low UV regime at 220 nm.
High Performance Liquid Chromatography (HPLC) Method for Analysis of 4-(2-Aminoethyl) benzenesulfonamide
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 220, 265 nm |
| Peak Retention Time | 5.21 min |
Description
| Class of Compounds | Amide |
| Analyzing Compounds | 4-(2-Aminoethyl) benzenesulfonamide |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Separation of 4-(2-Aminoethyl)benzenesulfonamide on Newcrom R1 HPLC column
May 16, 2018
4-(2-Aminoethyl)benzenesulfonamide can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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