HPLC Method for Analysis of  4-(2-Aminoethyl) benzenesulfonamide on Primesep 100  Column

HPLC Method for Analysis of 4-(2-Aminoethyl)benzenesulfonamide on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of  4-(2-Aminoethyl) benzenesulfonamide on Primesep 100  Column by SIELC Technologies

4-(2-Aminoethyl) benzenesulfonamide is an amide with the chemical formula C8H12N2O2S. When it is combined with hypoxic conditions, can inhibit the growth and induce apoptosis of human hepatocellular carcinoma cells. It is commonly used as an intermediate in synthesis of antidiabetic drugs. You can find detailed UV spectra of 4-(2-Aminoethyl)benzenesulfonamide and information about its various lambda maxima by visiting the following link.

4-(2-Aminoethyl)benzenesulfonamide can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) buffer. This analysis method can be detected in the low UV regime at 220 nm.

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 20/80%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 220, 265 nm
Peak Retention Time5.21 min

Description

Class of CompoundsAmide
Analyzing Compounds4-(2-Aminoethyl)benzenesulfonamide

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
4-(2-Aminoethyl)benzenesulfonamide
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.