| CAS Number | 66-40-0 |
|---|---|
| Molecular Formula | C8H20N |
| Molecular Weight | 130.254 |
| InChI Key | CBXCPBUEXACCNR-UHFFFAOYSA-N |
| LogP | -1.99 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Tetraethylammonium Chloride on Newcrom AH Column
July 11, 2023
HPLC Method for Analysis of Tetraethylammonium on Newcrom AH by SIELC Technologies
Tetraethylammonium is a quaternary ammonium cation with the chemical formula (C2H5)4N+. This compound consists of a central nitrogen atom surrounded by four ethyl groups.
As with other quaternary ammonium compounds, the nitrogen atom in tetraethylammonium carries a positive charge. This compound is often used in its salt forms, such as tetraethylammonium bromide or tetraethylammonium chloride.
Tetraethylammonium has a variety of uses in both the scientific and industrial domains. In biological research, it’s commonly used to block potassium channels in cell membranes, which allows researchers to study these channels in isolation. It’s also utilized in organic chemistry as a phase transfer catalyst, facilitating reactions between compounds in different phases.
The tetraethylammonium can be retained, analyzed, on a Newcrom AH column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium formate (AmFm) ionic modifier. This analysis method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).
LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tetraethylammonium
Condition
| Column | Newcrom AH, 4.6 x 150 mm, 3 µm, 100 A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | AmFm pH 3.0- 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50 °C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) (MS- compatible mobile phase) |
| Peak Retention Time | 7.12 min |
| Sample concentration | 1 mg/ml |
| Injection volume | 1 µl |
| LOD | 320 ppb |
Description
| Class of Compounds | Hydrophilic, Metal, Ion, Quaternary ammonium salt |
| Analyzing Compounds | Tetraethylammonium |
Application Column
Newcrom AH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Separation of Quaternary Amines
September 11, 2003
Primesep C separates a mixture of quaternary amines by a combination of cation exchange, complex formation, and hydrophic interactions. Methyltriethylammonium, tetraethylammonium, and tetramethylammonium cations as well as bromide counter ion are separated on a short 50 mm column. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and triethylamine acetate with evaporative light scattering detection (ELSD).
| Column | Primesep C, 4.6×50 mm |
| Mobile Phase | MeCN/H2O |
| Buffer | TEA acetate |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Quaternary Amines, Ionizable, Hormone |
| Analyzing Compounds | Methyltriethylammonium, tetraethylammonium, Tetramethylammonium |
Application Column
Primesep C
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsMethyltriethylammonium Bromide
Quaternary Amines
Tetraethylammonium
Tetramethylammonium
