Triazolam

Triazolam structural formula

CAS Number28911-01-5
Molecular FormulaC17H12Cl2N4
Molecular Weight343.210
InChI KeyJOFWLTCLBGQGBO-UHFFFAOYSA-N
LogP2.42
Synonyms
  • Triazolam
  • 8-Chloro-6-(2-chlorophenyl)-1-methyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine
  • 4H-[1,2,4]Triazolo[4,3-a][1,4]benzodiazepine, 8-chloro-6-(2-chlorophenyl)-1-methyl-
  • 28911-01-5
  • 4H-[1,2,4]Triazolo[4,3-a][1,4]benzodiazepine, 8-chloro-6-(2-chlorophenyl)-1-methyl-
  • 4H-[1,2,4]Triazolo[4,3-a][1,4]benzodiazepine,8-chloro-6-(2-chlorophenyl)-1-methyl-
  • 4H-s-Triazolo[4,3-a][1,4]benzodiazepine, 8-chloro-6-(o-chlorophenyl)-1-methyl-
  • 8-Chloro-1-methyl-6-(o-chlorophenyl)-4H-s-triazolo[4,3-a][1,4]benzodiazepine
  • Halcion
  • Novodorm
  • BRN 1226643
  • 8-Chloro-6-(o-chlorophenyl)-1-methyl-4H-s-triazolo(4,3-a)(1,4)benzodiazepine
  • 8-Chloro-6-(2-chlorophenyl)-1-methyl-4H-(1,2,4)triazolo(4,3-a)(1,4)benzodiazepine
  • Clorazolam
  • EINECS 249-307-3
  • Novidorm
  • Triazolamum
  • UNII-1HM943223R

Applications:

Uv-Vis Spectrum of Triazolam

February 16, 2026

Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Triazolam check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Triazolam
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Determination of Triazolam on Newcrom R1 Column

September 1, 2022

HPLC Method for Triazolam on Newcrom R1 by SIELC Technologies

HPLC Method for Determination of Triazolam on Newcrom R1 Column      

High Performance Liquid Chromatography (HPLC) Method for Analysis of Triazolam.

Triazolam is a prescription-strength benzodiazepine with the chemical formula C17H12Cl2N4. It is used to treat insomnia, as it is a central nervous system depressant tranquilizer. Compared to other benzodiazepines, it is fairly obscure, preventing misuse and abuse of it. It is a Schedule IV drug.

Triazolam can be retained and analyzed on a Newcrom R1 reverse-phase column using a simple isocratic analytical method with a mobile phase consisting of Acetonitrile (MeCN), water, and Sulfuric acid as the ionic modifier. This analysis method can be UV detected at 200 nm with high resolution and peak symmetry.

Condition
Column Newcrom R1, 2.1 x 100 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 35%
Buffer H2SO4 – 0.2%
Flow Rate 0.2 ml/min
Detection UV 210 nm

 

Description
Class of Compounds Drug
Analyzing Compounds Triazolam

Application Column

Newcrom R1

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Triazolam
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Triazolam on Newcrom R1 HPLC column

February 16, 2018
Separation of Triazolam on Newcrom C18 HPLC column

Triazolam can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Triazolam
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.