Trans-Resveratrol

CAS Number501-36-0
Molecular FormulaC14H12O3
Molecular Weight228.24
Synonyms
  • Resveratrol, 3,4?,5-Trihydroxy-trans-stilbene, 5-[(1E)-2-(4-Hydroxyphenyl)ethenyl]-1,3-benzenediol

Applications:

UV-Vis Spectrum of Trans-Resveratrol

July 15, 2024
UV-Vis Spectrum of trans-Resveratrol. Absorption Maxima: 216 nm, 306 nm.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.

Application Analytes:
Resveratrol
Trans-Resveratrol
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Trans – Resveratrol on Newcrom R1 Column

February 21, 2024

HPLC Method for Analysis of Trans-Resveratrol on Newcrom R1 Column by SIELC Technologies

Separation type: Liquid Chromatography Reversed-phase

HPLC Method for Analysis of Trans - Resveratrol on Newcrom R1 Column
HPLC Method for Analysis of Trans-Resveratrol on Newcrom R1 Column by SIELC Technologies

Trans-resveratrol is a natural compound that belongs to a class of polyphenolic compounds called stilbenes. Polyphenols are organic compounds found in plants and are known for their antioxidant properties. Resveratrol is specifically found in various plants, including the skin of red grapes, peanuts, and certain berries.

Isomers: Resveratrol exists in two main isomeric forms: trans-resveratrol and cis-resveratrol. The trans form is the more stable and biologically active isomer.

Antioxidant Properties: Resveratrol is known for its antioxidant activity, which means it can help neutralize free radicals in the body. Free radicals are unstable molecules that can contribute to oxidative stress and damage cells.

Potential Health Benefits: Resveratrol has been studied for its potential health benefits, including its role in cardiovascular health, anti-inflammatory effects, and potential anti-cancer properties. Some research suggests that resveratrol may have cardioprotective effects and could contribute to overall health.

Found in Red Wine: Red wine is often cited as a dietary source of resveratrol, as the compound is present in grape skins, and red wine is made by fermenting grape skins with the seeds and stems. However, the concentration of resveratrol in red wine is relatively low, and it would be challenging to consume enough through wine alone to achieve potential health benefits.

Supplements: Resveratrol supplements are also available and are marketed for their potential health-promoting properties. However, the effectiveness of these supplements and the optimal dosage for various health conditions are still subjects of ongoing research.

Trans – Resveratrol be retained and analyzed on a reversed-phase Newcrom R1 column with a mobile phase consisting of water, Acetonitrile (MeCN), and sulfuric acid. This analytical method can be detected with high resolution and peak symmetry at a wavelength of 265 nm using UV detection

High Performance Liquid Chromatography (HPLC) Method for Analyses of Trans-Resveratrol on Newcrom R1 Column by SIELC Technologies

Condition

ColumnNewcrom R1, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 30%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 305 nm
Injection volume:1 µL
Sample1 mg/ml
Sample DiluentMeCN/H2O – 50/50%
LOD* 5 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsPolyphenols
Analyzing CompoundsTrans-Resveratrol

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Trans-Resveratrol

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.