Theophylline monohydrate

Theophylline monohydrate structural formula

CAS Number5967-84-0
Molecular FormulaC7H10N4O3
Molecular Weight198.183
InChI KeyINQSMEFCAIHTJG-UHFFFAOYSA-N
LogP-0.0610
Synonyms
  • Theophylline monohydrate
  • 1,3-Dimethyl-3,7-dihydro-1H-purine-2,6-dione--water (1/1)
  • 1H-Purine-2,6-dione, 3,7-dihydro-1,3-dimethyl-, hydrate (1:1)
  • 5967-84-0
  • Dimethylxanthine
  • Somophyllin-CRT
  • Uni-Dur
  • 1,3-Dimethylxanthine monohydrate
  • Aerolate JR
  • Aerolate SR
  • Aquaphyllin
  • Elixomin
  • Elixophyllin SR
  • Quibron-T/SR
  • Theobid jr.
  • Theoclear L.A.-130
  • Theoclear L.A.-260
  • Theoclear-100
  • Theoclear-200
  • Theoclear-80
  • Theolair-SR
  • Theolixir
  • Theophylline 0.04% and dextrose 5% in plastic container
  • Theophylline 0.08% and dextrose 5% in plastic container
  • Theophylline 0.16% and dextrose 5% in plastic container
  • Theophylline 0.2% and dextrose 5% in plastic container
  • Theophylline 0.32% and dextrose 5% in plastic container
  • Theophylline 0.4% and dextrose 5% in plastic container
  • Theophylline and dextrose 5% in plastic container
  • Theophylline in dextrose 5% in plastic container
  • Theophylline-SR
  • Theophyl-SR
  • UNII-C137DTR5RG

Applications:

Separation of Theophylline monohydrate on Newcrom R1 HPLC column

May 16, 2018
Separation of Theophylline monohydrate on Newcrom R1 HPLC column

Theophylline monohydrate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Theophylline
Theophylline monohydrate
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.

Effect of Acid on Separation of Xanthines

July 6, 2003

Primesep B separates xanthines such as theobromine, dimethylxanthine, theophylline by a combination of reversed-phase and ion-exchange mechanisms. The anion-exchange properties of the column allow retention of hydrophilic xanthines, and the reversed-phase properties retain hydrophobic compounds. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either trifluoroacetic acid (TFA), perchloric acid (HClO4), or sulfuric acid (H2SO4) and UV detection.

Condition
Column Primesep B
Mobile Phase MeCN/H2O
Buffer TFA , HClO4, H2SO4
Flow Rate 1.0 ml/min
Detection

 

Description
Class of Compounds
 Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Theobromine, 1,7 – Dimethylxanthine, Theophyline

 

Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
1,7-Dimethylxanthine
Theobromine
Theophylline
Theophylline monohydrate
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.