| CAS Number | 126-83-0 |
|---|---|
| Molecular Formula | C3H6ClNaO4S |
| Molecular Weight | 196.581 |
| InChI Key | TZLNJNUWVOGZJU-UHFFFAOYSA-M |
| LogP | -2.32 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Sodium 3-chloro-2-hydroxypropane-1-sulfonate on Newcrom BH
January 17, 2022
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of sodium 3-chloro-2-hydroxypropane-1-sulfonate
Sodium 3-chloro-2-hydroxypropane-1-sulfonate is an anionic agent used to prepare negatively charged probe particles to investigate the nanoporosity of cellulosic fibers and their interactions with cationic polyelectrolytes.
Using a Newcrom BH mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, the constituent ions of sodium 3-chloro-2-hydroxypropane-1-sulfonate can be separated, retained, and analyzed. This analysis method can be detected by evaporative light scattering detection (ELSD). The ions can also be separated and retained using the same mobile phase, but with a sulfuric acid (H2SO4) buffer instead of the AmFm buffer. This method can be UV detected at 200 nm.
| Column | Newcrom BH, 3.2×100 mm, 3 µm, 100A |
| Mobile Phase | MeOH/H2O – 10/90% |
| Buffer | Ammonium Formate pH 3.0 – 40 mM |
| Flow Rate | 0.5 ml/min |
| Detection | ELSD |
| Column | Newcrom BH, 3.2×100 mm, 3 µm, 100A |
| Mobile Phase | MeOH/H2O – 10/90% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 200 nm |
| Class of Compounds | Sulfonate |
| Analyzing Compounds | Sodium 3-chloro-2-hydroxypropane-1-sulfonate |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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Separation of Sodium 3-chloro-2-hydroxypropane-1-sulfonate on Newcrom R1 HPLC column
February 16, 2018
Sodium 3-chloro-2-hydroxypropane-1-sulfonate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options