HPLC Determination of Sodium 3-chloro-2-hydroxypropane-1-sulfonate on Newcrom BH

Separation type: Liquid Chromatography Mixed-mode HPLC.cloud View on hplc.cloud   High Performance Liquid Chromatography (HPLC) Method for Analysis of sodium 3-chloro-2-hydroxypropane-1-sulfonate   Sodium 3-chloro-2-hydroxypropane-1-sulfonate is an anionic agent used to prepare negatively charged probe particles to investigate the nanoporosity of cellulosic fibers and their interactions with cationic polyelectrolytes. Using a Newcrom BH mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, the constituent ions of sodium 3-chloro-2-hydroxypropane-1-sulfonate can be separated, retained, and analyzed. This analysis method can be detected by evaporative light scattering detection (ELSD). The ions can also be separated and retained using the same mobile phase, but with a sulfuric acid (H2SO4) buffer instead of the AmFm buffer. This method can be UV detected at 200 nm.  

Condition 1

Column Newcrom BH, 3.2x100 mm, 3 µm, 100A
Mobile Phase MeOH/H2O - 10/90%
Buffer Ammonium Formate pH 3.0 - 40 mM
Flow Rate 0.5 ml/min
Detection ELSD
 

Condition 2

Column Newcrom BH, 3.2x100 mm, 3 µm, 100A
Mobile Phase MeOH/H2O - 10/90%
Buffer H2SO4 - 0.2%
Flow Rate 0.5 ml/min
Detection UV 200  nm

Description

Class of Compounds Sulfonate
Analyzing Compounds Sodium 3-chloro-2-hydroxypropane-1-sulfonate
 
Application Analytes:
Sodium 3-chloro-2-hydroxypropane-1-sulfonate