Quinolinic acid

HPLC Method for Analysis of Quinolinic acid on Newcrom BH Column by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

Quinolinic acid (also known as quinolinate or 2,3-pyridinedicarboxylic acid) is a dicarboxylic acid with a pyridine backbone. It plays a role in various biological processes and is associated with some pathological conditions.

Biochemical Role:

• Quinolinic acid is a metabolite in the kynurenine pathway, which is responsible for the catabolism of tryptophan, an essential amino acid.
• It serves as a precursor to nicotinamide adenine dinucleotide (NAD+), an essential coenzyme in cellular redox reactions.

Neurological Significance:

• Quinolinic acid can act as an excitotoxin in the brain, meaning it can overactivate nerve cells potentially leading to damage or death of the cells.
• High levels of quinolinic acid have been associated with various neurodegenerative disorders, such as Alzheimer’s disease, Huntington’s disease, and others, due to its ability to overstimulate neurons through the N-methyl-D-aspartate (NMDA) receptor.

Chemical Properties:

• Quinolinic acid has a somewhat polar character due to the carboxylic acid groups and the nitrogen in the pyridine ring.
• It is a weak acid and can lose protons from the carboxylic acid groups, forming salts in neutral and basic conditions.

Environmental Presence:

• Quinolinic acid, like other biologically active molecules, may be found in various environmental contexts, like in soil and water, through natural processes or as a result of anthropogenic activities, such as the use of pesticides that may contain pyridine derivatives.

Quinolinic acid can be retained and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and posphoric acid. This analytical method can detect compounds with high resolution and peak symmetry using UV detection at 200 nm

High Performance Liquid Chromatography (HPLC) Method for Analyses of Quinolinic acid

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