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For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. This analysis was done with the mobile phase made of 20% acetonitrile and 80% water.

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

Polyglutamic acid (PGA) is a biopolymer consisting of repeating units of Glutamic acid. PGA can be found and used in a wide variety of industries due to its biodegradable, non-toxic properties, including in the food, medical, and wastewater industries. Using SIELC’s newly introduced BIST™ method, PGA can be retained easily on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N’-Dimethylpiperazine (DMP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include TMDAP, Calcium acetate, and Magnesium acetate. Using this new and unique analysis method, PGA can be retained with high selectivity and great peak shape. This method can be detected and is compatible with ELSD, CAD, and Mass Spectrometry (LC-MS).

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Description

HPLC Method for Polyglutamic Acid on BIST A by SIELC Technologies

Polyglutamic acid (PGA) is a biopolymer consisting of repeating units of Glutamic acid. PGA can be found and used in a wide variety of industries due to its biodegradable, non-toxic properties, including in the food, medical, and wastewater industries. It has the chemical formula (C₅H₇NO₃)_n.

Using SIELC’s newly introduced BIST™ method, Polyglutamic Acid can be retained easily on a negatively-charged, cation-exchange BIST A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N’-Dimethylpiperazine (DMP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include TMDAP, Calcium acetate, and Magnesium acetate. Using this new and unique analysis method, PGA can be retained with high selectivity and great peak shape. This method can be detected and is compatible with ELSD, CAD, and Mass Spectrometry (LC-MS).

Condition

Description